Lab 4: Cryopreservation of cells
Embedded Files
Procedure
handwritten notes
Taking the cells out of the cryo storage container
Photo of me carefully taking the cells out of the cryo storage container to not get any liquid nitrogen on myself
Results
HEK 293 CELLS FLASK
Completed flask containing 0.5mL of cells and 10mL of MEM
Confluency
The confluency of the flask after 1 week was estimated to be at 70%
<-- cryocells flask
Flask containing the cryopreserved cells
<-- Cells post cryopreservation
The photo was taken two days after the cells were brought out of the cryo preservation container.
conclusion
In conclusion, the experiment provided valuable insight in cell preparation for cryopreservation and on the process of cryopreservation itself. The experiment was successful, reinforcing the already known findings in cryopreservation's importance. The cells used for this procedure can be split and used in future experiments.
Page updated
Google Sites
Report abuse