Lab 5: DNA transfection of cells
Embedded Files
Procedure
Handwritten Notes
Results
Well #1 before transfection
The photo was taken of well #1 after adding the 1.5 million cells. Even after trypsinizing, the confluency appears to be 95%
well #2 before transfection
The photo was taken of well #2 after adding the 1.5 million cells
<-- 6 well transfection plate
Photo of the 6 well plate containing the transfection mixture in wells 1 and 2
Well #1 post transfection 10x
Well #1 post transfection 20x
well #1 post transfection 20x
well #1 post transfection 10x
well #2 post transfection 10x
well #2 post transfection 20x
<-- 6 well plate post transfection
Blue colonies can be seen in the transfected wells
Conclusion
Based off the results, we can conclude the experiment was successful even with highly confluent wells before transfection. In the experiment, we successfully transferred exogenous DNA into the cells and got them to express it, even though the cell density was high. The experiment also demonstrated the effectiveness of the calcium phosphate method, providing valuable knowledge on an important process. This method in particular seems to be well liked due to the low costs associated.
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